Structure of lysozyme dissolved in neat organic solvents as assessed by NMR and CD spectroscopies

Autor: Alexander M. Klibanov, Tatyana Knubovets, John J. Osterhout
Rok vydání: 1999
Předmět:
Zdroj: Biotechnology and Bioengineering. 63:242-248
ISSN: 1097-0290
0006-3592
DOI: 10.1002/(sici)1097-0290(19990420)63:2<242::aid-bit13>3.0.co;2-n
Popis: The structure of the model protein hen egg-white lysozyme dissolved in water and in five neat organic solvents (ethylene glycol, methanol, dimethylsulfoxide (DMSO), formamide, and dimethylformamide (DMF)) has been examined by means of 1H NMR and circular dichroism (CD) spectroscopies. The NMR spectra of lysozyme reveal the lack of a defined tertiary structure in all five organic solvents, although the examination of line widths suggests the possibility of some ordered structure in ethylene glycol and in methanol. The near-UV CD spectra of the protein suggest no tertiary structure in lysozyme dissolved in DMSO, formamide, and DMF, while a distinctive (albeit less pronounced than in water) tertiary structure is seen in ethylene glycol and a drastically changed one in methanol. A highly developed secondary structure was observed by far-UV CD in ethylene glycol and methanol; interestingly, the alpha-helix content of the protein in both was greater than in water, while the beta-structure content was lower. (Solvent absorbance in the far-UV region prevents conclusions about the secondary structure in DMSO, formamide and DMF.)
Databáze: OpenAIRE
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