METHOD-DEPENDENT BIAS IN THE QUANTITATION OFCIS- ANDTRANS-RESVERATROL GLUCOSIDES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
| Autor: | Michael A. Levesque, Elisa Tsang, David M. Goldberg, George J. Soleas |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Chromatography Elution Clinical Biochemistry Pharmaceutical Science Glycoside Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Glucoside Polyphenol Quantitative analysis (chemistry) Cis–trans isomerism |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 22:1843-1855 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1081/jlc-100101770 |
| Popis: | The concentrations of cis and trans-polydatin (resveratrol 3-β-glucoside) were measured in 133 red wines by two methods: Method A utilized normal phase HPLC with isocratic elution followed by UV detection, and Method B was based on reverse phase HPLC with gradient elution and photodiode array detection. In each method, 20 μL of sample was directly injected without prior treatment. For both polydatin isomers, Method B gave higher values. These discrepancies were more marked with the second of two Calibrators prepared by purification of trans-polydatin from the dried roots of Polygonum cuspidatum. Overall, the correlation coefficients for the two methods were only 0.442 and 0.704 for the cis and trans isomers, respectively. No explanation for these findings was forthcoming. These results demonstrate the need for caution in interpreting and comparing values for polydatins reported using different methods, and emphasize the need for pure synthetic standards of these compounds to allow valid inter-laboratory a... |
| Databáze: | OpenAIRE |
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