| Autor: |
Elena Ramon, Antonia Anna Lettini, Mancin Marzia, M. C. dalla Pozza, Alessandra Longo, Antonia Ricci, Lisa Barco |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork. |
| Popis: |
The quality of DNA extract significantly influences the outcome of PCR-based detection methods. Performances of four different DNA extraction methods were evaluated for their ability to recover Salmonella DNA from artificially contaminated specimens. Swine faecal and pork samples were spiked with known concentration of Salmonella Typhimurium, DNA was then extracted by each of the methods considered and finally tested by a commercial Salmonella Real Time kit. The QIAgen and Adiafood kits were the most suitable methods to detect Salmonella in pig faeces. For pork samples the best performances were obtained using the Invitrogen kit. The use of appropriate DNA extraction methods is a critical issue for successful and valid PCR results and it is advisable that DNA extraction techniques are carefully selected with particular regard to the type of samples they should be used for. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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