Quantitative determination of adenosine tri- and diphosphates
| Autor: | I. F. Seits |
|---|---|
| Rok vydání: | 1957 |
| Předmět: |
chemistry.chemical_classification
Chemistry Phosphorus Inorganic chemistry chemistry.chemical_element General Medicine Adenosine General Biochemistry Genetics and Molecular Biology Quantitative determination Hydrolysis Adsorption Inorganic pyrophosphate ATP hydrolysis medicine Nucleotide medicine.drug |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 43:257-260 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf00786373 |
| Popis: | The specificity and conditions of the application of the determination method of adenosine tri- and adenosine diphosphates by means of nucleotides adsorption on coal (method developed by Crane and Lipmann) have been studied. It has been stated that the presence in samples, besides ATP and ADP, of fructose-1, 6-diphosphate, fructose-6-phosphate, glucose-1-phosphate, inorganic pyrophosphate, orthophosphate, adenylic acid and DPN does not interfere with the determination of ATP and ADP. Completeness of ATP adsorption depends upon the pH of the medium, as well as upon the ratio between the adsorbed substance and the adsorbent. Acidity corresponding to 4–5% trichloracetic acid and the ratio 100 mg of coal per 100 γ of readily hydrolyzed phosphorus of ATP are the most favorable conditions. This method is simple, specific, and accurate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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