The nucleotide sequence of phenylalanine tRNA from Mycoplasma sp. (Kid)

Autor:	Dieter Söil, Kwok S. Szeto, Margaret E. Kimball
Rok vydání:	1974
Předmět:	chemistry.chemical_classification Nucleic acid sequence Phenylalanine Mycoplasma Biology medicine.disease_cause Molecular biology Amino acid chemistry Biochemistry Transfer RNA Genetics medicine Protein biosynthesis Nucleotide Escherichia coli
Zdroj:	Nucleic Acids Research. 1:1721-1732
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/1.12.1721
Popis:	Phenylalanine tRNA from Mycoplasma sp. (Kid) was purified and characterized. The tRNA can be aminoacylated by phenylalanyl-tRNA synthetase from both Mycoplasma and E. coli. In a tRNA-dependent cell-free E. coli amino acid incorporating system programmed with poly U pure Mycoplasma tRNAPhe was fully active in promoting phenylalanine incorporation, even in direct competition with homologous E. coli tRNAPhe. Since the Mycoplasma tRNA lacks isopentenyladenosine, or any related hypermodified nucleoside, it appears that the presence of such nucleosides in tRNA is not an absolute requirement for protein synthesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1761586539ea045cdc1aab70410d272f https://doi.org/10.1093/nar/1.12.1721 Zobrazit plný text záznamu