Progress in the Generation of Multiple Lineage Human-iPSC-Derived 3D-Engineered Cardiac Tissues for Cardiac Repair
| Autor: | Shuji Setozaki, William J. Kowalski, Fei Ye, Fangping Yuan, Bradley B. Keller, Marc Dwenger, Hidetoshi Masumoto, Takeichiro Nakane, Joseph P. Tinney |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Ejection fraction Lineage (genetic) business.industry Cell behavioral disciplines and activities Embryonic stem cell In vitro Mural cell medicine.anatomical_structure Internal medicine mental disorders Cardiac repair medicine Cardiology business Induced pluripotent stem cell |
| Zdroj: | Molecular Mechanism of Congenital Heart Disease and Pulmonary Hypertension ISBN: 9789811511844 |
| DOI: | 10.1007/978-981-15-1185-1_54 |
| Popis: | Despite improvements over the past 30 years, 10-year pediatric heart transplantation survival rates remain low and mechanical support is both expensive and relatively unavailable. In contrast to isolated cell therapies, implantable engineered cardiac tissues (ECTs) recover myocardial mass and function, creating the opportunity for cardiac recovery rather than replacement. Our ECT research has progressed from using embryonic avian and rodent cell compositions to human induced pluripotent stem cell (h-iPSC)-derived, multiple cell lineage formulations with the goal of clinical translation. We generate ECTs from h-iPSC-derived cardiomyocytes (CM), endothelial cells (EC), and vascular mural cells (MC) in both linear (15 × 1 mm) and large format (LF, 20 × 20 mm) geometries using rodent-derived and human-compatible reagents. H-iPSC ECTs undergo rapid gel compaction and begin intrinsic beating by day 3. CM fraction at ECT formation is approximately 60%. The ECT maximum capture rates increased during in vitro culture up to 28 days and in response to optogenetic pacing (OP) using AAV-ChIEF from day 7 to 14. ECT relaxation times decreased, force-frequency relations became more neutral, and beat-to-beat hysteresis decreased with prolonged culture or OP. H-iPSC-derived ECTs generated using human collagen I and human compatible MaxGel have comparable structural and functional features to rodent-derived ECTs. Linear and LF-ECTs implanted onto xenotolerant infarcted rat hearts survived, engrafted, improved ejection fraction, normalized regional echo strain, and reduced scar area at 4 weeks. Thus, these h-iPSC ECT compositions show promise as a strategy for pediatric myocardial recovery. |
| Databáze: | OpenAIRE |
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