Application of Raman Spectroscopy for Studying the Mechanisms of Neutrophil Activation by Carbon Nanotubes
| Autor: | Tatsiana Kulahava, G. N. Semenkova, Mikhail V. Shuba, Yu. S. Leonik, Lena Golubewa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Extracellular Traps Phagocytosis chemistry.chemical_element Carbon nanotube Condensed Matter Physics Oxygen law.invention chemistry.chemical_compound symbols.namesake chemistry law Biophysics symbols Cytoskeleton Raman spectroscopy Spectroscopy DNA |
| Zdroj: | Journal of Applied Spectroscopy. 88:77-84 |
| ISSN: | 1573-8647 0021-9037 |
| DOI: | 10.1007/s10812-021-01143-1 |
| Popis: | Raman spectroscopy was used to determine mechanisms for neutrophil activation upon interaction with multi-walled carbon nanotubes (MWCNTs) possessing CO2H groups (MWCNTCO2H) and in complexes with DNA (MWCNT–CO2H/DNA). Short MWCNT–CO2H and MWCNT–CO2H/DNA (100–300 nm) were found to interact with neutrophils, causing cytoskeleton reorganization, namely, activation of G-actin to give F-actin in cells. Raman spectroscopy showed that these CNTs are not phagocytized by neutrophils. MWCNT–CO2H enhances the generation of reactive oxygen and chlorine species by neutrophils as indicated by an increase in the NADPH–oxidase activity in a cell suspension. The interaction of MWCNT–CO2H/DNA leads to inhibition of the oxygen-activating ability of phagocytes and formation of neutrophilic extracellular traps (NET) with the inclusion of carbon nanostructures. The free radical products generated by neutrophils disrupt the crystal structure of MWCNTs. |
| Databáze: | OpenAIRE |
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