| Autor: |
Tingwei Zhu, Peipei Fan, Qianyu Li, Luyan Ma, Qian Wei, Xingfeng Guo, Fusheng Chen |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-1859866/v1 |
| Popis: |
Previous study found soybean isolate protein (SPI)-based composite films added by corn starch (CS) (SPI:CS (4:1) composite film, SPI:CS (1:1) composite film) exhibited good mechanical property and better stability during the short-term storage pre-experiment. These properties were related to internal structure changes. In order to accordingly understanding the causes driving stability changes in performance and then controlling long-term stability of the SPI-based composite films, plasticizer emigration and structural properties of SPI-based composite films stored at different temperatures (5 ℃, 20 ℃, 35 ℃) and 54% relative humidity were systematically investigated over a period of time. High storage temperature (＞ 20 ℃) accelerated plasticizer emigration which reflected in increased migration rate of glycerol molecules and decreased moisture content. During the storage of SPI-based composite films, the decreased free SH partly formed new disulfide bonds and non-covalent inter-molecular interactions like hydrogen bonds changes significantly weakened the protein structure, which influenced mechanical properties changes during storage. PAGE and FTIR results explained the protein aggregation was bond with disulfide bonds and the reorganization of protein structure with prevalence of extended β-sheet conformation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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