Survey of the Incidence of Viral Infections in Calanthe spp. and Characterization of a GW Isolate of Cymbidium mosaic virus in Korea
| Autor: | Jeong Seon Kim, Da Some Baek, Chung Youl Park, Jonghee Oh, Jong Yoon Choi, Su Heon Lee, Gil Hun Jang, Dae Hyeon Bae |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cymbidium mosaic virus Calanthe Odontoglossum ringspot virus viruses Nucleic acid sequence Nicotiana benthamiana Plant Science Biology biology.organism_classification Biochemistry Virology Genome Virus Cucumber mosaic virus 03 medical and health sciences 030104 developmental biology Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | Research in Plant Disease. 22:65-71 |
| ISSN: | 1598-2262 |
| DOI: | 10.5423/rpd.2016.22.2.65 |
| Popis: | Cymbidium mosaic virus (CymMV) is a major virus infecting orchid plants and causing economic loss. In this study, the incidence of viral infection in Calanthe spp. at the Korean Institute of Calanthe was investigated using reverse transcription polymerase chain reaction. The CymMV infection rate was 42%, and the two viruses Odontoglossum ringspot virus and Cucumber mosaic virus had frequencies of 8% and 2%, respectively. Additionally, we characterized an isolate of CymMV, CymMV-GW, using biological tests and examined the nucleotide sequence properties of its complete genome. CymMVGW induced chlorotic ringspots and chlorotic spot symptoms in inoculated leaves of Chenopodium amaranticolor and Nicotiana benthamiana, respectively. In this study, we have for the first complete genome sequence of CymMV-GW in Korea. The CymMV-GW genome was 6,225 nucleotides in length, excluding the poly-(A) tail, and showed whole-genome nucleotide and amino acid sequence identities of 97.7% and 100%, respectively, with the NJ-1 isolate of CymMV. Here, we report the complete genome sequence of the CymMV-GW isolate and viral infection rates for Calanthe spp. in Korea. |
| Databáze: | OpenAIRE |
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