Cryo-electron microscopy reveals macromolecular organization within biological liquid cyrstals seen in the polarizing microscope
| Autor: | A.G. Fowler, F.P Booy |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Polarized light microscopy
Materials science Cryo-electron microscopy Scanning confocal electron microscopy General Medicine Biochemistry Dark field microscopy law.invention Crystallography Electron diffraction Structural Biology law Energy filtered transmission electron microscopy Electron microscope Bacterial virus Molecular Biology |
| Zdroj: | International Journal of Biological Macromolecules. 7:327-335 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/0141-8130(85)90047-9 |
| Popis: | A method is described for examining water dispersible biopolymers in the frozen, hydrated state by electron microscopy using the filamentous bacterial viruses Pf1 and fd as examples. The technique reveals liquid-crystalline textures that correlate well with polarizing microscopy of magnetically oriented specimens. At higher magnification the packing of the virus particle is revealed to a spatial resolution of better than 30 A, thus linking directly with data from X-ray diffraction and optical microscopy. Electron diffraction confirms that the structure is preserved to high resolution (4 A). The technique permits a detailed understanding of the processes involved in the orientation of these samples in a strong magnetic field and clarifies the long-range bi-axial properties of some fibres as seen by X-ray diffraction and optical microscopy. |
| Databáze: | OpenAIRE |
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