Extracellular Adenosine 5'-Triphosphate Decreases the Permeability of an Endothelial Cell Monolayer through Protein Kinase C

Autor:	Atsuo Murata, Taichi Takeda, Syuji Shimazaki
Rok vydání:	2000
Předmět:	Histology Physiology Kinase Cell Biology Biology Biochemistry Adenosine Molecular biology Pathology and Forensic Medicine Endothelial stem cell chemistry.chemical_compound chemistry Extracellular Phorbol medicine Staurosporine Signal transduction Protein kinase C medicine.drug
Zdroj:	ACTA HISTOCHEMICA ET CYTOCHEMICA. 33:103-107
ISSN:	1347-5800 0044-5991
DOI:	10.1267/ahc.33.103
Popis:	Adenosine 5′−triphosphate (ATP) is known to have various effects on cells as an extracellular nucleotide. It decreases the degree of edema and the requirement for intravenous hydration in critically ill patients. We examined the direct effects of ATP on the permeability of a vascular endothelial cell monolayer using an in vitro co−culture chamber system. Human umbilical vein endothelial cells were cultured on filter membranes of co−culture chambers to form a confluent monolayer. FITC-labeled 70k dalton dextran and ATP at various concentrations were added to the upper chamber. The concentrations of FITC−dextran, which permeated to the lower chamber through the endothelial cell monolayer, were determined in 4hr. A significant decrease in the permeability of FITC−dextran was observed with 10-6 to 10-3M of ATP. One, 10, 100, and 1000μM ATP decreased the permeability from 1.00±0.05 to 0.75±0.05, 0.66±0.04, 0.61±0.07, and 0.58±0.08 respectively (mean±SD, n=5, p
Databáze:	OpenAIRE
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