Increasing DNA Transfer Efficiency by Temporary Inactivation of Host Restriction
| Autor: | R A Helm, Stanley Maloy, Robert Edwards |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
biology
Electroporation Genetic transfer biology.organism_classification medicine.disease_cause General Biochemistry Genetics and Molecular Biology law.invention Microbiology Transduction (genetics) chemistry.chemical_compound chemistry law medicine Recombinant DNA Escherichia coli Bacteria In vitro recombination DNA Biotechnology |
| Zdroj: | BioTechniques. 26:892-900 |
| ISSN: | 1940-9818 0736-6205 |
| Popis: | E. coli and Salmonella typhimurium are widely used bacterial hosts for genetic manipulation of DNA from prokaryotes and eukaryotes. Introduction of foreign DNA by electroporation or transduction into E. coli and Salmonella is limited by host restriction of incoming DNA by the recipient cells. Here, we describe a simple method that temporarily inactivates host restriction, allowing high-frequency DNA transfer. This technique might be readily applied to a wide range of bacteria to increase DNA transfer between strains and species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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