BMDREDDREGULATES THE APOPTOSIS COORDINATING WITHBMDAXX,BMCIDE-B,BMFADD, ANDBMCREBINBMN CELLS
Autor: | Deng-pan Zhang, Yan Lu, Hu-hu Xin, Yun-gen Miao, Mei-xian Wang, Peng Jiao, Rui-ting Chen, Shuang Liang |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Programmed cell death biology Physiology fungi General Medicine CREB Biochemistry Molecular biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Death-associated protein 6 Apoptosis RNA interference 030220 oncology & carcinogenesis Insect Science biology.protein FADD Caspase Death domain |
Zdroj: | Archives of Insect Biochemistry and Physiology. 93:160-173 |
ISSN: | 0739-4462 |
Popis: | The apoptosis mechanisms in mammals were investigated relatively clearly. However, little is known about how apoptosis is achieved at a molecular level in silkworm cells. We cloned a caspase homologous gene named BmDredd (where Bm is Bombyx mori and Dredd is death-related ced-3/Nedd2-like caspase) in BmN cells from the ovary of Bm and analyzed its biological information. We constructed the N-terminal, C-terminal, and overexpression vector of BmDredd, respectively. Our results showed that the transcriptional expression level of BmDredd was increased in the apoptotic BmN cells. Furthermore, overexpression of BmDredd increased the caspase-3/7 activity. Simultaneously, RNAi of BmDredd could save BmN cells from apoptosis. The immunofluorescence study showed that BmDredd located at the cytoplasm in normal cell otherwise is found at the nucleus when cells undergo apoptosis. Moreover, we quantified the transcriptional expressions of apoptosis-related genes including BmDredd, BmDaxx (where Daxx is death-domain associated protein), BmCide-b (where Cide-b is cell death inducing DFF45-like effector), BmFadd (Fadd is fas-associated via death domain), and BmCreb (where Creb is cAMP-response element binding protein) in BmN cells with dsRNA interferences to detect the molecular mechanism of apoptosis. In conclusion, BmDredd may function for promoting apoptosis and there are various regulatory interactions among these apoptosis-related genes. |
Databáze: | OpenAIRE |
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