Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR
| Autor: | Hui Wang, Jun Han, Weixiang Hong, Mai Zhang, Yuting Jia, Zhengping Li, Honghong Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Messenger RNA DNA ligase Chemistry Hybridization probe Metals and Alloys General Chemistry Molecular biology Catalysis Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention law RNA splicing Materials Chemistry Ceramics and Composites Splice junction splice Ligation Polymerase chain reaction |
| Zdroj: | Chemical Communications. 57:10011-10014 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/d1cc03033g |
| Popis: | A method based on mRNA-templated ligation of splice-junction anchored DNA probes followed by PCR amplification of the ligated product has been developed for multiplexed detection of mRNA splice variants with high sensitivity and specificity. The proposed assay can detect as low as 10 aM mRNA splicing variants and has been successfully applied to detect real samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|