Thin-Layer Chromatography—Densitometry and Thin-Layer Chromatography—Image Analysis for Screening Bile Acid-Binding Activities of Thai Edible Plants

Autor:	Apirak Sakunpak, Namfa Sermkaew, Tossaton Charoonratana, Natawat Chankana, Pathamaporn Pathompak, Jirapornchai Suksaeree
Rok vydání:	2015
Předmět:	Chloroform Chromatography Bile acid medicine.drug_class Silica gel Clinical Biochemistry Bile acid binding Biochemistry Thin-layer chromatography Analytical Chemistry chemistry.chemical_compound Acetic acid chemistry Bile acid sequestrant medicine Densitometry
Zdroj:	JPC - Journal of Planar Chromatography - Modern TLC. 28:380-385
ISSN:	1789-0993 0933-4173
DOI:	10.1556/1006.2015.28.5.6
Popis:	Despite the fact that many researchers have developed different methods for the determination and detection of bile acid sequestrant, the analytical instruments are still quite costly, and expertise is usually required. In order to avoid the above-mentioned problems, simple, rapid, effective, and inexpensive methods to evaluate bile acid-binding are essential. The purpose of this study was to develop a simple and rapid thin-layer chromatography (TLC)—image analysis method to quantitatively determine the bile acid-binding activity of ten edible ethanolic plant extracts. The method was carried out by using TLC silica gel 60 F254 plate with the mobile phase composed of chloroform, methanol, and acetic acid (7:2:1 v/v). The chromatograms were analyzed with image analysis software (ImageJ). The method provided good linearity, accuracy, reproducibility, and selectivity for the determination of bile acid-binding activity. Litsea petiolata extract was found to show the most potent bile acid-binding activity compa...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::16d5661328e518e981e3e6c52b998865 https://doi.org/10.1556/1006.2015.28.5.6 Zobrazit plný text záznamu Full text from SpringerLink