| Popis: |
Although it has been shown that red cell viability can be maintained for at least two years by storage in glycerol at −79° C, there is a progressive loss of viability after six months of storage at −20° C. Because of the practical objections to storage at −79° C, studies have been directed at the efficacy of storage at −45° C and at possible causes for the progressive deterioration observed during storage at −20° C. The in vivo survival of cells stored up to 18 months at −45° C is as good as for fresh cells. In addition, the damaging effect of oxygenation of red cells prior to storage at −20° C is described, as well as the prevention of this damage by the addition of anti-oxidants (glutathione, ascorbic acid) to the red cell suspension. The in vivo survival of red cells stored for one year at −20° C in the presence of ascorbic acid and glycerol is poor. |
