Popis: |
To improve the detection of ovulation and early diagnosis of pregnancy on dairy farms, a disposable electrochemical immunosensor was developed using indirect competitive immunoassays and screen-printed carbon electrodes. After optimisation of the assay conditions, the linear range was from 0.16 to 50 ng/mL. The limit of detection was 0.16 ng/mL. There was no cross-reactivity with testosterone, 17-β-estradiol and estriol. The intra-assay coefficient of variation was less than 13% and interassay coefficient of variation was |