High-Yield Laccase-Producing Strains Constructed by Protoplast Fusion Between Bacterium and Fungus
| Autor: | Wenli Yin, Lihong Zhao, Lele Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Laccase
biology Strain (chemistry) Biomedical Engineering Biophysics Bioengineering Environmental pollution Cellulase Protoplast biology.organism_classification medicine.disease_cause Biochemistry Microbiology medicine biology.protein Pleurotus ostreatus Food science Escherichia coli Bacteria Biotechnology |
| Zdroj: | The Open Biotechnology Journal. 9:221-224 |
| ISSN: | 1874-0707 |
| DOI: | 10.2174/1874070701509010221 |
| Popis: | This study is based on the construction of high-yield laccase-producing fusant achieved by inter-kingdom protoplast fusion between Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditions were demonstrated with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants were screened for different characteristics between two parental strains and further identified by laccase activity, offering one of the genetically stable fusants, Strain F. The fusant F produced the highest yield of laccase, being about 22% higher than that of the parental strain. The results suggest that the protoplast fusion technique can be considered as a promising technique for the control of environmental pollution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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