N-Linked Oligosaccharide Processing Enzymes as Molecular Targets for Drug Discovery

Autor:	Tadatake Oku, Haruhiro Okuda, Hiroyuki Osada, Kiyoshi Fukuhara, Wataru Hakamata, Makoto Muroi, Masaaki Kurihara, Toshiyuki Nishio, Akira Takatsuki
Rok vydání:	2006
Předmět:	chemistry.chemical_classification biology Drug discovery Oligosaccharide chemistry.chemical_compound Enzyme Biosynthesis chemistry Biochemistry Cell culture biology.protein Secretion Glycoprotein Glucosidases
Zdroj:	Journal of Applied Glycoscience. 53:149-154
ISSN:	1880-7291 1344-7882
DOI:	10.5458/jag.53.149
Popis:	N-Linked oligosaccharide processing enzymes are key enzymes in the biosynthesis of N-linked oligosaccharides. These enzymes are a molecular target for inhibition by anti-viral agents that interfere with the formation of essential glycoproteins required in viral assembly, secretion and infectivity. We think that the molecular recognition of three kinds of glucosidases (family 13 and family 31 α-glucosidases and endoplasmic reticulum glucosidases) are different. Therefore, glycon and aglycon specificity profiling of glucosidases was an important approach for the research of glucosidase inhibitors. We carried out the profiling of glucosidases using small molecules as a probe. Moreover, we designed and synthesized three types of glucosidase inhibitors. These compounds were evaluated with regard to their ability to inhibit glucosidases in vitro, and were also tested in a cell culture system. We found some compounds having glucosidase inhibitory activity and anti-viral activity.
Databáze:	OpenAIRE
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