The Effect of 9-cis-Retinoic Acid on Proliferation and Differentiation of A Spermatogonia and Retinoid Receptor Gene Expression in the Vitamin A-Deficient Mouse Testis**This work was supported by the Netherlands Organization for Scientific Research through GB-MW Grant 900-544-101 (to A.M.M.v.P.)
| Autor: | Dirk G. de Rooij, Bianca H. G. J. Schrans, Paul T. van der Saag, E. Sonneveld, Axel P. N. Themmen, Ans M.M. van Pelt, I. C. Gaemers |
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| Rok vydání: | 1998 |
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| Zdroj: | Endocrinology. 139:4269-4276 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.139.10.6272 |
| Popis: | Retinoid X receptors (RXRs) are key regulators in retinoid signaling. Knowledge about the effects of 9-cis-retinoic acid (9-cis-RA), the natural ligand for the RXRs, may also provide insight in the functions of RXRs. In this study, the effect of 9-cis-RA on spermatogenesis in vitamin A-deficient (VAD) mice was examined. Administration of 9-cis-RA stimulated the differentiation and subsequent proliferation of the growth-arrested A spermatogonia in the testis of VAD mice. However, compared with all-trans-retinoic acid (ATRA), relatively higher doses of 9-cis-RA were necessary. This could not simply be due to a lower or delayed activity of 9-cis-RA, as simultaneous administration of ATRA and 9-cis-RA did not cause a synergistic effect. Instead, the presence of 9-cis-RA diminished the effect of ATRA by approximately one third. Studies of in vivo transport and metabolism showed that ATRA and 9-cis-RA, after administration to VAD mice, penetrated the testis equally well. However, 9-cis-RA was metabolized much f... |
| Databáze: | OpenAIRE |
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