SAS™ Molecular Tests Salmonella Detection Kit
| Autor: | Chandra Bapanpally, Sharon L Brunelle, Akif Kasra, Shah Khan, Laura Montier |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pharmacology
Salmonella biology Hold time Loop-mediated isothermal amplification food and beverages biology.organism_classification medicine.disease_cause Analytical Chemistry Food and drug administration Ground turkey medicine Environmental Chemistry Spinach Food science Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 97:808-819 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.13-326 |
| Popis: | The SAS™ Molecular tests Salmonella Detection method, a Loop-mediated Isothermal Amplification method, performed as well as or better than the U.S. Department of Agriculture-Food Safety Inspection Service Microbiology Laboratory Guidebook and the U.S. Food and Drug Administration Bacteriological Analytical Manual reference methods for ground beef, beef trim, ground turkey, chicken carcass rinses, bagged mixed lettuce, and fresh spinach. The ground beef (30% fat, 25 g test portion), poultry matrixes and leafy greens were validated in a 6–7 h enrichment, and ground beef (30% fat, 375 g composite test portion) and beef trim (375 g composite test portion) were validated in a 16–20 h enrichment. The method performance for meat and leafy green matrixes was shown to be acceptable under conditions of co-enrichment with Escherichia coli O157. Thus, after a short 6–7 h co-enrichment step, ground beef, beef trim, lettuce, and spinach can be tested for both Salmonella and E. coli O157. Inclusivity and exclusivity testing revealed no false negatives and no false positives among the 100 Salmonella serovars and 30 non- Salmonella species examined. The method was shown to be robust when enrichment time, DNA extract hold time, and DNA volume were varied. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|