Conjugal plasmid transfer inStreptomycesresembles bacterial chromosome segregation by FtsK/SpoIIIE
| Autor: | Günther Muth, York-Dieter Stierhof, Jutta Vogelmann, Constanze Finger, Jamil Guezguez, Matthias Flötenmeyer, Dirk Linke, Moritz Ammelburg, Wolfgang Wohlleben |
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| Rok vydání: | 2011 |
| Předmět: |
Transfer DNA
Genetics General Immunology and Microbiology biology General Neuroscience Circular bacterial chromosome Streptomyces coelicolor Trab Bacterial genome size biology.organism_classification General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Plasmid chemistry Horizontal gene transfer Molecular Biology DNA |
| Zdroj: | The EMBO Journal. 30:2246-2254 |
| ISSN: | 0261-4189 |
| DOI: | 10.1038/emboj.2011.121 |
| Popis: | Conjugation is a major route of horizontal gene transfer, the driving force in the evolution of bacterial genomes. Antibiotic producing soil bacteria of the genus Streptomyces transfer DNA in a unique process involving a single plasmid-encoded protein TraB and a double-stranded DNA molecule. However, the molecular function of TraB in directing DNA transfer from a donor into a recipient cell is unknown. Here, we show that TraB constitutes a novel conjugation system that is clearly distinguished from DNA transfer by a type IV secretion system. We demonstrate that TraB specifically recognizes and binds to repeated 8 bp motifs on the conjugative plasmid. The specific DNA recognition is mediated by helix α3 of the C-terminal winged-helix-turn-helix domain of TraB. We show that TraB assembles to a hexameric ring structure with a central ∼3.1 nm channel and forms pores in lipid bilayers. Structure, sequence similarity and DNA binding characteristics of TraB indicate that TraB is derived from an FtsK-like ancestor protein, suggesting that Streptomyces adapted the FtsK/SpoIIIE chromosome segregation system to transfer DNA between two distinct Streptomyces cells. |
| Databáze: | OpenAIRE |
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