Ultrahistochemischer Nachweis der Blutgruppensubstanz B menschlicher Erythrocyten mit einem Lectin aus Salmo gairdneriRich

Autor:	Werner Linss, Zimmermann N, Herwart Feuerstein, Ernst Reuter
Rok vydání:	1988
Předmět:	Histology Blood group substance biology Chemistry Gold labelling Lectin Cell Biology General Medicine Biochemistry Polyclonal antibodies Labelling biology.protein Human erythrocytes Fish Protein A
Zdroj:	Acta Histochemica. 84:101-105
ISSN:	0065-1281
DOI:	10.1016/s0065-1281(88)80019-9
Popis:	Summary From the roe of Salmo gairdneri Rich ., a lectin was isolated which agglutinates specifically human erythrocytes of blood group B. For cytochemical labelling of the blood group substance B on the surface of human erythrocytes, an indirect approach was chosen. By means of a polyclonal antibody from the rabbit against the B-specific fish lectin, electronmicroscopic presentation was performed with Protein A gold using a multistep method. For quantification gold labelling was partially followed by a silver technique.
Databáze:	OpenAIRE
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