New chimeric promoter useful for expression of selectable marker genes in rice transformation

Autor:	Hiromi Higo, Hironori Mano, Kayo Tsuruya, Yuzo Minobe, Kana Hasegawa
Rok vydání:	2005
Předmět:	Reporter gene Transgene fungi food and beverages Plant Science Biology Molecular biology Genetically modified rice Marker gene Transformation (genetics) Enhancer Agronomy and Crop Science Gene Selectable marker Biotechnology
Zdroj:	Plant Biotechnology. 22:287-294
ISSN:	1347-6114 1342-4580
DOI:	10.5511/plantbiotechnology.22.287
Popis:	We developed a new chimeric promoter to express a selectable marker gene specifically at the selection stage during transformation of rice (Oryza sativa L.). The promoter consists of a 135-bp upstream sequence of the rice RezA gene and 4 or 8 copies of an enhancer fragment of the CaMV35S promoter. The β-glucuronidase (GUS) reporter gene under the control of this promoter was strongly expressed in transgenic rice calli but not in endosperm, the edible tissue in rice grains. When the hygromycin B phosphotransferase gene was fused with this chimeric promoter and introduced into rice, we were able to select transgenic plants. We demonstrated that the chimeric promoter did not influence expression of the endogenous RezA gene in the transgenic calli. These results indicate that this chimeric promoter could be useful for the selection of transgenic rice free of marker gene products in the edible tissue.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::16848c0c5c3356b8d3a4c1a150572045 https://doi.org/10.5511/plantbiotechnology.22.287 Zobrazit plný text záznamu