| Popis: |
Gamma-tocotrienol has been reported to exhibit a protective effect on hepatocarcinogenesis through the enhancement of immune response and anti-proliferation effect on breast cancer cell lines such as MDA-MB-435 and MCF7. The anti-proliferation mechanism was suggested to be via an increase in apoptosis. The effects of palm oil γ-tocotrienol and α-tocopherol on the cell proliferation were determined using 5-Bromo-2’-deoxy-uridine (BrdU) detection method. CaSki and Alexander cells were sensitive to γ-tocotrienol and α-tocopherol-anti proliferation effects with 93.5 to 97.8% (P less than 0.01, n is equal to 4) and 59.7 to 69.1% (P less than 0.001, n is equal to four) inhibition, respectively beginning with a concentration of 100μM and above. Treatment with α-tocopherol showed a lesser inhibition in the proliferation activity of CaSki cell by 19.7 to 39.4% (P less than 0.01, n is equal to four) and Alexander cell by 16.9 to 19.6% (P less than 0.001, n is equal to four) beginning with a concentration of 50μM and 200μM, respectively. However, both the compounds had no effect on Chang cell. Gamma-tocotrienol showed an IC50 inhibition of CaSki and Alexander cells growth at a dose of 75μM (P less than 0.05, n is equal to four) and 66μM (P |
