Cytotoxic and apoptotic activity of nitrofuroxans on lymphoma cells

Autor:	Sergey N. Kolomeichuk, Igor V. Ovchinnikov, Yakov P. Nizhnik, Nina N. Makhova
Rok vydání:	2018
Předmět:	0301 basic medicine medicine.diagnostic_test Chemistry Organic Chemistry medicine.disease Molecular biology Staining Raji cell Lymphoma Flow cytometry 03 medical and health sciences 030104 developmental biology Apoptosis hemic and lymphatic diseases P53 status Fluorescence microscope medicine Cytotoxic T cell
Zdroj:	Chemistry of Heterocyclic Compounds. 54:70-75
ISSN:	1573-8353 0009-3122
DOI:	10.1007/s10593-018-2232-z
Popis:	Five nitrofuroxans were synthesized and evaluated for their in vitro cytotoxicity against human lymphoma cells with different p53 status (Raji Burkitt's lymphoma and MOLT-4). The most promising compound exhibited IC50 values of 2.6–11.0 μM in MTT growth inhibitory assays. Flow cytometry data suggest that cytotoxic effects of given compounds in MOLT-4 cells are mediated by apoptosis via a p53-dependent pathway. Changes in nuclear morphology after treatment were evaluated by fluorescent microscopy after Hoechst staining. However, the studied nitrofuroxans did not induce apoptosis in Raji cells having the p53 mutant gene, thus suggesting a different mechanism of their action.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::167475fd21c0b168a163b17fa3eeaa26 https://doi.org/10.1007/s10593-018-2232-z Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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