Regulation of chimeric phosphoenolpyruvate carboxykinase genes by the trans-dominant locus TSE1
| Autor: | Robin J Leach, Mathew J. Thayer, R. E. K. Fournier, T. G. Lugo |
|---|---|
| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | Molecular and Cellular Biology. 10:2660-2668 |
| ISSN: | 1098-5549 0270-7306 |
| Popis: | Extinction of phosphoenolpyruvate carboxykinase (PCK) gene expression in hepatoma x fibroblast hybrids is mediated by a trans-acting genetic locus designated tissue-specific extinguisher 1 (TSE1). To identify PCK gene sequences required for extinction, hepatoma transfectants expressing PCK-thymidine kinase (TK) chimeric genes were fused with TK- fibroblasts and PCK-TK expression in the resulting hybrids was monitored. Expression of a PCK-TK chimera containing PCK sequences between base pairs -548 and +73 was extinguished in four of five hepatoma transfectants tested, although hybrids derived from one transfectant clone failed to extinguish PCK-TK expression. In contrast, crosses between hepatoma transfectants expressing the herpesvirus TK gene from its own promoter and TK- fibroblasts produced TK+ hybrids; extinction of the transfected TK gene was not observed. Thus, rat PCK gene sequences between base pairs -548 and +73 are sufficient for tissue-specific extinction in hybrid cells. Extinction of PCK-TK gene expression in transfectant microcell hybrids mapped specifically to human chromosome 17, the site of human TSE1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|