Characterisation of phytoplasma (16SrVI) associated with little leaf disease ofPortulaca grandiflora– Anin silicoanalysis
| Autor: | Bharat Gopalrao Somkuwar, Abdul Samad, Mahesh K. Gupta, P. V. Ajayakumar, S. Panda |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Genetics
Biology 16S ribosomal RNA biology.organism_classification law.invention Terminal restriction fragment length polymorphism Restriction enzyme law Phytoplasma Restriction fragment length polymorphism Agronomy and Crop Science Ribosomal DNA Nested polymerase chain reaction Polymerase chain reaction |
| Zdroj: | Archives Of Phytopathology And Plant Protection. 44:1447-1455 |
| ISSN: | 1477-2906 0323-5408 |
| DOI: | 10.1080/03235408.2010.505779 |
| Popis: | A new severe little leaf disease was observed on P. grandiflora, popular as Moss-rose Purslane, widely grown in temperate zones. Characteristic symptoms, ultrastructural studies, antibiotic response and amplification of 16S ribosomal DNA fragments (about 1.5 kb) by polymerase chain reaction (PCR) from infected samples, suspect the involvement of phytoplasma as a pathogen. Nested PCR product, 1.2 kb, with primer pairs R16F2n/R16R2 used for cloning and sequencing. Comparision of the 16S rRNA gene sequences showed that the causal, PLL phytoplasma, is very close (98%) to Indian brinjal little leaf (EF186820) and “Candidatus Phytoplasma trifolii” (AY390261), 16SrVI group phytoplasmas, previously reported from India and Canada respectively. Here, the status of PLL (EF651786) is verified by computer-simulated restriction fragment length polymorphism analysis of 16S rRNA genes of the F2n/R2 sequences of closely related strains of the 16SrVI group using 17 restriction enzymes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|