Point mutation in the TGFBI gene: surface-enhanced infrared absorption spectroscopy (SEIRAS) as an analytical method
| Autor: | M. A. Ríos-Corripio, F. J. Villalobos-Piña, J. R. Molina-Contreras, Oscar F. Chacon-Camacho, D. Rosas-Vara, Raul Ayala-Ramirez, C. Frausto-Reyes, Juan Carlos Zenteno, Beatriz Buentello-Volante, R. Hernández-Martínez |
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| Rok vydání: | 2019 |
| Předmět: |
Mutation
Chemistry General Chemical Engineering Point mutation Infrared spectroscopy Nanoparticle 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease_cause 01 natural sciences Biochemistry Industrial and Manufacturing Engineering 0104 chemical sciences Colloidal gold Materials Chemistry Biophysics medicine Surface plasmon resonance Fourier transform infrared spectroscopy 0210 nano-technology Spectroscopy |
| Zdroj: | Chemical Papers. 74:1079-1086 |
| ISSN: | 1336-9075 2585-7290 |
| Popis: | A novel method to detect DNA mutations based on gold nanoparticles is described. This bioconjugate was prepared by conjugation of the TGFBI gene on the surface of gold nanoparticles. The surface plasmon resonance band observed in the ultraviolet–visible spectrum of the gold nanoparticles showed a shift to a lower energy after the surface was covered with the TGFBI gene. Surface-enhanced infrared spectroscopy (SEIRAS) showed the absorption bands of the bioconjugate due to their proximity to the nanoparticles. The SEIRAS effect was able to detect small and specific changes in the sequence of the TGFBI gene using interactions of the bioconjugates. The study was performed on patients clinically diagnosed with lattice corneal dystrophy (LCD). To our knowledge, this is the first report of FTIR spectroscopy employed to analyse this gene. We used genotyping results previously obtained by next-generation DNA sequencing technology to ensure that the analysed samples had the mutation. Our results show highly reproducible signals, which could be used for analytical studies of mutations in the genome. |
| Databáze: | OpenAIRE |
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