| Autor: |
P. Marescaux, M. Lhermitte, E. Belan, M. Goudemand, N. Houdret |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Journal of Liquid Chromatography. 17:2171-2177 |
| ISSN: |
0148-3919 |
| Popis: |
Clomipramine and its N-demethylated metabolite, N-demethyl-clomipramine were determined simultaneously in plasma or in erythrocytes by a simple hexane-diethyl ether-step extraction procedure followed bv reversed-phase liquid chromatography. Baseline separation was achieved by a 5-μm cyanopropylsilane column. The mobile phase consisted of 10mM phosphate buffer-methanol-acetonitrile (23:15:60, v/v/v), at a flow rate of 1.2 ml/min. The eluant was monitored by a UV detector operating at 220 nm. The inter- and intra assay coefficients of variation were within 3.6 and 6.0% for clomipramine and 4.1 and 6.5 % for N-demethyl clomipramine. respectively. The lowest limit of detection of clomipramine and N-demethylated clomipramine was 5 ng/ml. The method is sensitive, specific and allows for routine analysis in therapeutic controls of patients treated by this antidepressant. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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