Ciliary Mechanism of Regulating Hedgehog and Wnt/β-Catenin Signaling Modulates Ultraviolet B Irradiation-Induced Photodamage in HaCaT Cells
| Autor: | Yong Miao, Kai Gao, Tianding Shen, Xue Wang, Zhiqi Hu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
biology
Chemistry Photoaging 0206 medical engineering Biomedical Engineering Wnt signaling pathway Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Proinflammatory cytokine Cell biology HaCaT Downregulation and upregulation GLI1 Ciliogenesis medicine biology.protein General Materials Science 0210 nano-technology Hedgehog |
| Zdroj: | Journal of Biomedical Nanotechnology. 15:196-203 |
| ISSN: | 1550-7033 |
| DOI: | 10.1166/jbn.2019.2664 |
| Popis: | Skin undergoes photoaging, typically characterized by UV-damage lesions with matrix degradation and inflammatory changes. This process involves Hedgehog (Hh) signaling and the canonical Wnt pathway. Recently, primary cilia-microtubule-based organelles-have been implicated in the transduction of Hh and Wnt signaling. However, their role in photoaging remained unclear. In this study, we have shown that UVB causes both upregulation of MMPs and proinflammatory cytokines, as well as cilia formation in HaCaT cells, and that these changes can be attenuated by ciliogenesis inhibitor HPI-4 and anti-inflammatory PD98059 treatment. These cellular processes involved downregulation of transcriptional activity of components of Hh signaling, Gli1 and Gli2, as well as the turnover of p-β-catenin in canonical Wnt signaling, indicating the ciliary mechanism in photoaging. Our data demonstrate for the first time that pharmaceutical targeting of the primary cilia might have therapeutic benefits in the treatment of photoaging. |
| Databáze: | OpenAIRE |
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