Trichomonas vaginalis Enfeksiyonu Tanısında Mikroskobik İnceleme, Kültür ve Polimeraz Zincir Reaksiyonu Testlerinin Değerlendirilmesi
| Autor: | Levent Donmez, Dilek Colak, Dilara Ogunc, Hatice Yazisiz, Sibel Gümüş, Özlem Koyuncu Özyurt, Betil Özhak, Murat Ozekinci, Gözde Öngüt, Feryal Öztürk Eryiğit |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Gynecology Vaginal discharge 0303 health sciences medicine.medical_specialty Trichomoniasis General Immunology and Microbiology 030306 microbiology business.industry Becton dickinson medicine.disease_cause medicine.disease 03 medical and health sciences Infectious Diseases Trichomonas Vaginitis medicine Outpatient clinic Trichomonas vaginalis Nugent score medicine.symptom Bacterial vaginosis business |
| Zdroj: | Mikrobiyoloji Bulteni. 54:135-143 |
| ISSN: | 0374-9096 |
| Popis: | Sexually transmitted infections (STIs) are important as a public health problem all over the world. There are some difficulties in prevention and control programs of STIs due to clinical and laboratory diagnostic problems.The most common STIs are Chlamydia trachomatis infections, trichomoniasis and gonorrhea. The study aimed to investigate the direct microscopic examination, culture and polymerase chain reaction (PCR) tests in the diagnosis of Trichomonas vaginalis infection; to determine other microbiological agents that may cause vaginal discharge and to evaluate the various social variables in women with vaginal discharge admitted to the outpatient clinic of Obstetrics and Gynecology in Akdeniz University Hospital. Two hundred and fifteen patients were enrolled in the study. The socio-demographic features of the patients were recorded. Vaginal/endocervical swab specimens taken from patients were evaluated by microscopic examination. Swab specimens were inoculated into blood agar, MacConkey agar and chocolate agar for bacterial culture. Modified Trichosel broth with 5% horse blood (Becton Dickinson, USA) was used for Trichomonas spp. culture. The presence of C.trachomatis, Neisseria gonorrhoeae, and T.vaginalis in swab samples were investigated by multiplex PCR assay (BD Max CT/GC/TV, Becton Dickinson, USA). At least one pathogen was detected among 65 (30.3%) samples. T.vaginalis was detected by microscopic examination and PCR in four of 215 (1.9%) patients. Existence of yeast morphology was observed in 21 (9.8%) specimens by microscopic examination. Twenty four (11.2%) patients were diagnosed as bacterial vaginosis microscopically according to Nugent score system. Candida species grew in 32 (14.9%) and Streptococcus agalactiae grew in 2 (0.9%) of the specimens. C.trachomatis was detected in 2 (0.9%) samples and N.gonorrhoeae in 1 (0.5%) sample by PCR. In this study, 95.3% of the patients were married and 96.7% had only one sexual partner in the mean time. The rate of detection of pathogens were statistically higher in women who have had two or more pregnancies (p |
| Databáze: | OpenAIRE |
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