| Autor: |
S. Avnstrøm, O. J. Bjerrum, M. Wilken, T. Plesner, N. Tinggaard, A. W. Boyd, G. F. Burns, T. N. Buican, G. W. Hoffmann, L. Chatenoud, M. Baudrihaye, G. Goldstein, J. F. Bach, E. Clark, R. Holly, T. Yokochi, A. C. Feller, M. R. Parwaresch, H. H. Wacker, K. Lennert, F. M. Hofman, E. Yanagihara, B. A. Byrne, R. Billing, S. Baird, D. Frisman, C. R. Taylor, F. K. Jansen, H. Blythman, D. Carrière, P. Casellas, I. Hellström, K. E. Hellström, O. Gros, P. Gros, J. C. Laurent, Ph. Poncelet, G. Richer, I. Royston, H. Vidal, G. Voisin, C. Kieda, M. Monsigny, M. Waxdal, R. Kurrle, W. Lang, E. J. Kanzy, Th. Hofstaetter, F. R. Seiler, T. Matejić, G. Terzić, K. Isaković, B. D. Janković, H. J. Radzun, F. Spring, D. Anstee, H. Stein, J. Gerdes, U. Schwab, H. Lemke, A. Tabilio, W. Vainchenker, U. Testa, G. Vinci, J. Guichard, J. P. Vernant, P. Mannoni, J. Breton-Gorius, M. Teodorescu, S. Ralapati, A. van Es, H. J. Tanke, W. M. Baldwin, P. J. Oljans, J. S. Ploem, L. A. van Es |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Leucocyte Typing ISBN: 9783642688591 |
| DOI: |
10.1007/978-3-642-68857-7_77 |
| Popis: |
Sixteen proteins were demonstrated by crossed immunoelectrophoresis (CIE) of Triton X-100 solubilized megakaryoblasts against a rabbit antiserum. One of these reacted with a recently developed monoclonal antibody (SA-1) prepared by immunization with cells from a patient with megakaryoblastic leukemia. Leukemic cells from 8/15 patients with AML stained brightly for this antigen by indirect immunofluorescence. The antiserum also stained a minor subpopulation of myelo-monocytic cells in blood and bone-marrow of healthy individuals, but not lymphoblasts from 5 patients with ALL or cells from 5 CLL patients and 4 non-Hodgkin lymphomas. The protein was shown to be amphiphilic (integral) by charge-shift CIE and hydrophobic interaction CIE with phenyl-Sepharose. It was shown not to be glycosylated by Lentil lectin affinity CIE and by Sodiumborohydride labelling. SDS-polyacrylamide gel electrophoresis of 125I (Bolton-Hunter) labelled, immunoprecipitated material demonstrated 2 bands of molecular weight 34.000 and 46.000 under reducing and non-reducing conditions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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