| Popis: |
The mammalian heart is a muscular organ that can adjust dynamic alterations in its workload. In response to physiological or pathological stimuli and development, the heart changes hypertrophic enlargement which increase in the size of individual cardiac myocytes. A comparative proteomic survey is needed to acquire systemic insights into mammalian cardiac hypertrophy. In this study, using a shotgun proteomics approach based on MudPIT and tandem mass spectrometry, we have gained global protein profiling for each of pathological and physiological cardiac hypertrophy models and applied a statistical method by normalization of spectral counts. We analyzed relative protein expression abundance and studied their biological functions with bioinformatics tools. Interestingly, between physiological and pathological cardiac hypertrophy there were little overlap in the expression profiles. More specifically, there was a significant, negative correlation in biological functions of two models. Thus, different models of pathological and physiological cardiac hypertrophy are associated with distinct proteins controlled each signaling pathway. These data demonstrate key biological functions which describe each type of cardiac hypertrophy. |
