Use of Total Fluorescence Spectroscopy for the Highly Sensitive Simultaneous Determination of Fluoroquinolones in Rabbit Plasma

Autor:	José A. Murillo Pulgarín, N. Boras, A. Alañón Molina
Rok vydání:	2021
Předmět:	Matrix (chemical analysis) Emission scan Analyte Chromatography Sarafloxacin Chemistry Flumequine medicine Emission spectrum Fluorescence Fluorescence spectroscopy Analytical Chemistry medicine.drug
Zdroj:	Journal of Analytical Chemistry. 76:733-741
ISSN:	1608-3199 1061-9348
DOI:	10.1134/s1061934821060101
Popis:	A method for the quantification of a mixture of fluorescent antibiotics with broad, highly overlapped excitation and emission spectra is proposed. The method enables the simultaneous determination of sarafloxacin, flumequine and ofloxacin by using the fluorescence excitation-emission matrix and performing two different scans, namely, a linear variable angle synchronous fluorimetry scan at 15.6° and an emission scan at λexc of 320 nm. This ensures complete resolution of the mixture without the need for a separation technique. Various factors influencing the fluorescence intensity were assessed in order to maximize the sensitivity and obtain an adequate selectivity. The method was successfully used to determine the analytes in pharmaceuticals and rabbit plasma. The results obtained compared favorably with those provided by HPLC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::161d86d818fda6dfd4c30b3ff5e61411 https://doi.org/10.1134/s1061934821060101 Zobrazit plný text záznamu Full text from SpringerLink