Morphological and Physiological Changes Associated with Desiccation in Maize Embryos

Autor: J. S. Burris, D. S. Feng, J. M. Peterson, A. J. Perdomo
Rok vydání: 1997
Předmět:
Zdroj: Basic and Applied Aspects of Seed Biology ISBN: 9789401064101
DOI: 10.1007/978-94-011-5716-2_12
Popis: This study proposes to further clarify the effect of drying temperature and rate on vigour, membrane integrity, mitochondrial competence and synthetic processes in susceptible genotypes. Hybrid maize of the seed parent B73 by H99xH95 as pollen parent was harvested periodically, husked and dried under four temperature regimes to 12% mc providing drying times from 4 to 300 h. Standard germination results were generally high and cold test values were similar regardless of temperature or rate. Dry weights from seedlings grown at 25°C were responsive to drying temperature and rate while cold test dry weights showed little difference except for the rapid drying treatment in which root development was consistently depressed more than shoot. Membrane integrity was not compromised in seed dried in normal thinlayer drying but the rapidly dried material exhibited a linear increase in leakage with time. Oxygen consumption by imbibing embryos was reduced by high drying temperature but not rate. Incomplete alignment of lipid bodies along the cell wall following rapid drying treatments as compared to complete alignment in all others suggests roles in both drying and imbibition. Percentage of a 66 kDa protein (associated with maturation) in proportion to total non heat-denatured protein increased in response to drying but not at the rapid rate.
Databáze: OpenAIRE