| Popis: |
Ecotoxicological studies have been using tissular biomarkers to assess the effects of environmental and pollution stressors. The aim of this study was to conduct a health diagnosis using tissular biomarkers in the liver and muscle of Scomberomorus cavalla. Ten organisms were collected to obtain their morphometric and biological parameters from which a status index was calculated. Fish tissues were analyzed with histological and immunohistochemical (metallothioneins and Hsp70 antibodies) techniques. The tissue samples (25 from the liver and 43 from muscles) were stained with H-E, selecting biological responses for the immunochemical biomarker analysis. The total average of weight and length was 83 cm and 3400 g, respectively; status index was 0.6, suggesting an allometric relationship and that development has not been affected. Only one organism showed parasites in the liver; this organ presented hepatocytes with its nucleus and blood vessels with nucleated erythrocytes; some of the lesions found were focal inflammation, infiltration, granulomas, and eosinophilic secretions. The muscle presented packages enclosed in connective tissue, peripheral ovoid nuclei, and striations. The connective tissue showed blood vessels with oval nucleated erythrocytes. The antigen-antibody reaction with metallothionein in the liver and muscle was negative; Hsp70 was focally positive in the hepatocyte cytoplasm and negative in the muscle. Liver biomarker showed evidence of reversible damage, while the muscle’s morphologic and biochemical integrity is not at risk. In conclusion, S. cavalla captured in Tecolutla, Ver., does not show important effects due to environmental or pollution stress and can be considered mostly healthy. |
