A novel highly sensitive electrophoretic method for the detection and quantification of proteinase activity

Autor:	Otto M. Poulsen, Jann Hau
Rok vydání:	1986
Předmět:	chemistry.chemical_classification Chromatography Clinical Biochemistry Sodium Caseinate Penetration (firestop) Biochemistry Analytical Chemistry Highly sensitive Hydrolysis chemistry.chemical_compound Electrophoresis Enzyme chemistry Proteinase activity Agarose
Zdroj:	Electrophoresis. 7:166-171
ISSN:	1522-2683 0173-0835
DOI:	10.1002/elps.1150070404
Popis:	A novel highly sensitive method for the detection and quantification of proteinase activity is presented. The proteinases were forced electrophoretically into the substrate (sodium caseinate) containing agarose gel. The formation of enzyme-substrate complexes retarded the migration of the proteinases in the electric field and decreased the overall mobility of the enzymes. The proteinases were released as a result of the degradation of the substrate, and consequently the height of the obtained rocket shaped zones of hydrolysis were proportional to the concentration of proteinases in the samples. The electrophoretic method accelerated penetration of the substrate-containing gel by the proteinases and subsequently resulted in a 100-fold increase in sensitivity as compared to the radial diffusion method, depending entirely on diffusion.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::160c7fb38d4963e07fbe2380dc8d38a5 https://doi.org/10.1002/elps.1150070404 Zobrazit plný text záznamu Plný text