Modified lead hematoxylin-tartrazine yellow stain highlights changes in aortic wall after balloon angioplasty
| Autor: | Carl S. Hoegler, Charlene F Blando‐Hoegler |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Tunica media Aorta Histology Chemistry H&E stain Connective tissue Anatomy 030204 cardiovascular system & hematology Tunica intima Balloon Stain Staining 03 medical and health sciences Medical Laboratory Technology 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure medicine.artery cardiovascular system medicine |
| Zdroj: | Journal of Histotechnology. 41:140-148 |
| ISSN: | 2046-0236 0147-8885 |
| DOI: | 10.1080/01478885.2018.1483548 |
| Popis: | Hematoxylin and eosin (H&E) has been traditionally used to highlight the morphology of elastic arteries. An alternative staining procedure, lead hematoxylin-tartrazine yellow (LHTY), will be compared with H&E to evaluate its depiction of various layers in rat aortic wall. Sliced aortic rings, from 1-year-old male Sprague–Dawley rats that had undergone balloon angioplasty, were harvested at 1 day, 7 days, and 14 days after this procedure. Stained sections of specimen rings were evaluated to determine the effects on cellular components and extracellular matrix of various sublayers in the three aortic tunics. In contrast to H&E, the LHTY staining was found to clearly differentiate sublayers of the tunica intima from the tunica media. In addition, the elastic lamellar bands in the tunica media were especially demarcated to provide greater visibility for obtaining measurable indices (such as thickness). Moreover, LHTY-stained rings clearly depicted connective tissue elements within the intervening subl... |
| Databáze: | OpenAIRE |
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