Determination of selected antiepileptic drugs in mouse brain homogenates by HPLC—DAD
| Autor: | Karol Wróblewski, Anna Petruczynik, S. J. Czuczwar, Monika Waksmundzka-Hajnos, I. Radzik |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Diethylamine
Chromatography 010401 analytical chemistry Extraction (chemistry) General Chemistry Chloroformate Carbamazepine 030226 pharmacology & pharmacy 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Distilled water medicine Methanol Acetonitrile medicine.drug |
| Zdroj: | Acta Chromatographica. 29:219-234 |
| ISSN: | 2083-5736 1233-2356 |
| DOI: | 10.1556/1326.2017.29.2.6 |
| Popis: | Three independent reversed phase high-performance liquid chromatography (HPLC) procedures with diode array detection (DAD) for the analysis of carbamazepine (CBZ), topiramate (TPM), and valproic acid (VPA) have been developed in order to determine drug penetration of the blood—brain barrier. Determination of CBZ was performed on C18 column with mobile phases containing methanol (55%, v/v), acetate buffer at pH 3.5 (20%, v/v), double distilled water (25%, v/v), and 0.025 M L−1 diethylamine (DEA). The mobile phase containing acetonitrile and water (8:2, v/v) or acetonitrile and phosphate—citrate buffer at pH 2.6 (1:1), respectively, for analysis of VPA and TPM was applied. Quantification of carbamazepine was performed at 285 nm without extraction procedure before the analysis. Determination of topiramate and valproic acid was performed using precolumn derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl). FMOC-Cl is a suitable agent, which reacts with both primary and secondary amines and also with ... |
| Databáze: | OpenAIRE |
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