Caught in the Loop: Binding of the [Ru(phen) 2 (dppz)] 2+ Light‐Switch Compound to Quadruplex DNA in Solution Informed by Time‐Resolved Infrared Spectroscopy

Autor:	Frederico R. Baptista, Igor V. Sazanovich, Thorfinnur Gunnlaugsson, Fergus E. Poynton, Christine J. Cardin, John M. Kelly, Stephen J. Devereux, Michael Towrie, Susan J. Quinn
Rok vydání:	2020
Předmět:	010405 organic chemistry Light switch Guanine Organic Chemistry Stacking Infrared spectroscopy General Chemistry 010402 general chemistry 01 natural sciences Catalysis 0104 chemical sciences Thymine Nucleobase chemistry.chemical_compound Crystallography chemistry Intramolecular force Binding site
Zdroj:	Chemistry – A European Journal. 26:17103-17109
ISSN:	1521-3765 0947-6539
DOI:	10.1002/chem.202002165
Popis:	Ultrafast time-resolved infrared (TRIR) is used to report on the binding site of the [Ru(phen)2 (dppz)]2+ "light-switch" complex with both bimolecular (Oxytricha nova telomere) and intramolecular (human telomere) guanine-quadruplex structures in both K+ and Na+ containing solutions. TRIR permits the simultaneous monitoring both of the "dark" and "bright" states of the complex and of the quadruplex nucleobase bases, the latter via a Stark effect induced by the excited state of the complex. These data are used to establish the contribution of guanine base stacking and loop interactions to the binding site of this biologically relevant DNA structure in solution. A particularly striking observation is the strong thymine signal observed for the Na+ form of the human telomere sequence, which is expected to be in the anti-parallel conformation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::15f567a57e43f46c9dc847510794fe19 https://doi.org/10.1002/chem.202002165 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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