Region-Specific Regulation of Cytochrome P450 Aromatase Messenger Ribonucleic Acid by Androgen in Brains of Male Rhesus Monkeys1
| Autor: | Charles E. Roselli, Ada Celia Pereyra-Martinez, John A. Resko, Henry L. Stadelman |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
medicine.medical_specialty Messenger RNA biology medicine.drug_class Central nervous system Cell Biology General Medicine urologic and male genital diseases Androgen medicine.anatomical_structure Endocrinology Reproductive Medicine Hypothalamus Internal medicine Dihydrotestosterone medicine biology.protein Aromatase Gonadotropin hormones hormone substitutes and hormone antagonists Testosterone medicine.drug |
| Zdroj: | Biology of Reproduction. 62:1818-1822 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod62.6.1818 |
| Popis: | We demonstrated previously that testosterone regulates aromatase activity in the anterior/dorsolateral hypothalamus of male rhesus macaques. To determine the level of the androgen effect, we developed a ribonuclease protection assay to study the effects of testosterone or dihydrotestosterone (DHT) on aromatase (P450AROM) mRNA in selected brain areas. Adult male rhesus monkeys were treated with testosterone or DHT. Steroids in serum were quantified by RIA. Fourteen brain regions were analyzed for P450AROM mRNA. Significant elevations of its message over controls (P < 0.05) were found in the medial preoptic area/anterior hypothalamus of both androgen treatment groups and the medial basal hypothalamus of the testosterone-treated males. Other brain areas were not affected by androgen treatment. We conclude that testosterone and DHT regulate P450AROM mRNA in brain regions that mediate reproductive behaviors and gonadotropin release. The P450AROM mRNA of other brain areas is not androgen dependent. Bra... |
| Databáze: | OpenAIRE |
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