Synthesis and in silico and in vitro evaluation of trimethoxy-benzamides designed as anti-prion derivatives

Autor: Carolina F. Goes, Maria Claudia Barbosa, Lucas M. Ascari, Anderson S. Pinheiro, Yraima Cordeiro, Raissa Alves da Conceição, Rodolfo C. Maia, Carolina O. Matos, Natália Ferreira, Marina Amaral Alves, Alessandra Mendonça Teles de Souza, Byron Caughey
Rok vydání: 2019
Předmět:
Zdroj: Medicinal Chemistry Research. 28:2128-2141
ISSN: 1554-8120
1054-2523
DOI: 10.1007/s00044-019-02441-2
Popis: Transmissible spongiform encephalopathies (TSEs), also known as prion diseases, are neurodegenerative disorders which affect mammals, including the human species, and arise after the conversion of the monomeric cellular prion protein (PrPC) into the aggregated scrapie form (PrPSc). There is no therapy to treat TSEs and the identification of compounds that bind PrPC, preventing its conversion into PrPSc, is a viable therapeutic strategy. We designed and synthesized six novel trimethoxy-benzamide compounds as anti-prion drug candidates. Molecular docking analyses predicted that all the derivatives bind to a hotspot region located in the PrP globular domain with very similar spatial orientation and interaction mode. Although none of the analogs inhibited in vitro-aggregation of recombinant PrP (rPrP) in a cell-free conversion assay, the RT-QuIC, compound 8a accelerated rPrP conversion into PrPSc-like species. STD-NMR and ITC analyses indicated that both 8a and 8b bind to rPrP90–231. These analogs were toxic to PrPSc-infected cell lines, hence we could not assess their anti-prion activity by using this cellular approach, although this toxicity was cell line-dependent. These results point out that the 4-amino-quinoline trimethoxy-benzamide scaffold described herein represents a novel chemical pattern useful as a starting point for future structural optimization in the design of PrP ligands with improved affinity and safety profiles.
Databáze: OpenAIRE
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