Examination of the Adsorption of Large Biological Molecules to Anion Exchange Surfaces Using Surface Plasmon Resonance
| Autor: | William Riordan, Kurt Brorson, Mark R. Etzel, Scott Lute |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Ion exchange viruses Process Chemistry and Technology General Chemical Engineering Biomolecule Kinetics Analytical chemistry Filtration and Separation General Chemistry chemistry.chemical_compound Adsorption Reaction rate constant Membrane chemistry Chemical physics Surface plasmon resonance DNA |
| Zdroj: | Separation Science and Technology. 45:1-10 |
| ISSN: | 1520-5754 0149-6395 |
| DOI: | 10.1080/01496390903401770 |
| Popis: | Separation of viruses and other contaminants from protein therapeutics using anion exchange membrane adsorbers is a successful new approach to viral clearance; however, the fundamental phenomena that control performance are not well understood. For example, the kinetics of adsorption to the anion exchange surface may limit clearance, but has yet to be characterized experimentally and mathematically. In the present study, surface plasmon resonance was used to determine the adsorption kinetics for five large biological molecules: phage PP7, phage ΦX174, phage PR772, thyroglobulin, and DNA. Rate constants were incorporated into a kinetic model of chromatography to illustrate the impact on performance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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