| Autor: |
Tao Qin, Jia Kun Dai, Sheng Yun Xu, Ya Hui Wei, Qi Ma, Hui Hui Zhuang, Xi Chen, Yan Li |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Advanced Materials Research. :191-195 |
| ISSN: |
1662-8985 |
| DOI: |
10.4028/www.scientific.net/amr.998-999.191 |
| Popis: |
An appropriate ultrasonication of collecting foreign recombinant protein (human-adiponectin) from yeast was established. Regarded the bioactivity of foreign recombinant protein tested by Western Blotting analysis as parameter, a L9(33) orthogonal array design based on a series of single-factor experiments was employed to optimize conditions for ultrasonic disruption. The results showed that the ultrasonic power of 450 W, duration time on 25 min and operation interval (work time: intermittent time) of 10:10 (s/s) were optimal process with the highest protein bioactivity, and meanwhile, the cell breaking rate was (67.8±2.1) %. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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