Influence of Different Types of Block Copolymers on the Secondary Structure and Stability of the Staphylolytic Lysk Enzyme
| Autor: | I. A. Novozhilov, N. G. Balabushevich, L.Y. Filatova, Natalia L. Klyachko, V. G. Pugachev, David M. Donovan, T. A. Chubar |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification technology industry and agriculture Cationic polymerization General Chemistry Polymer Polyethylene glycol 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Enzyme chemistry Chemical engineering Polylysine Copolymer Protein secondary structure |
| Zdroj: | Moscow University Chemistry Bulletin. 73:179-184 |
| ISSN: | 1935-0260 0027-1314 |
| DOI: | 10.3103/s0027131418040028 |
| Popis: | A branched block copolymer of polyethyleneimine and polyethylene glycol (PEI–PEG) and a linear block copolymer of polylysine and polyethylene glycol (PLL–PEG) form complexes with the staphylolytic LysK enzyme. The complexation between LysK and cationic block-copolymers results in a 1.5–2.0-fold increase in the lytic activity of LysK and a 2–20-fold increase in its half-life. The stabilizing effects of the block copolymers depend on the temperature, the NaCl concentration, and the polymer/enzyme ratio. Being an effective stabilizer, linear PLL–PEG provides an increase in the LysK stability suitable for biomedical materials (LysK maintains 100% of its activity after four months at 4°C). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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