PD09-08 INTRAPERITONEAL ADMINISTRATION OF MESENCHYMAL STEM CELLS IS EFFECTIVE IN PREVENTING DETRUSOR DYSFUNCTION AFTER PARTIAL BLADDER OUTLET OBSTRUCTION

Autor:	Rutuja Kadam, Jennifer Carleton, Bridget Waife, Adetola B Adesida, Peter Metcalfe, Tom Churchill
Rok vydání:	2019
Předmět:	medicine.medical_specialty business.industry Urology medicine.medical_treatment Mesenchymal stem cell medicine.disease Bladder outlet obstruction Cytokine Mechanism of action Downregulation and upregulation Fibrosis medicine Smooth muscle hypertrophy medicine.symptom business PI3K/AKT/mTOR pathway
Zdroj:	Journal of Urology. 201
ISSN:	1527-3792 0022-5347
Popis:	INTRODUCTION AND OBJECTIVES:Mesenchymal stem cells (MSC) have shown great promise with respect to the prevention and treatment of detrusor dysfunction and fibrosis after partial bladder outlet obstruction (pBOO). Our lab has previously demonstrated short and long-term benefits with a reduction in the inflammatory and hypoxic cytokine cascade, decreased smooth muscle hypertrophy, prevention of fibrosis, and improvement in urodynamic parameters. Although our intravenous administration has demonstrated uptake of MSC into the bladder, we hypothesize the primary mechanism of action is via a systemic downregulation of the inflammatory and hypoxic cascade. Therefore, we repeated the pBOO in our model using the intraperitoneal (IP) administration of MSC to compare the efficacy to intravenous administration.METHODS:5x106 MSC via IP had similar effects to 106 MSC IV, while 106 IP was not as effective. 5 x106 IP showed greater reduction in TGF-B1, MTOR, and Col 1+3 than 106 IV (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::15aa85dfe8b71d882d82d6682d1d2572 https://doi.org/10.1097/01.ju.0000555266.36014.0f Zobrazit plný text záznamu