Detecting Antibodies Secreted by Trapped Cells Using Extraordinary Optical Transmission
| Autor: | Karen L. Kavanagh, Bonnie L. Gray, Sean F. Romanuik, Alexandre G. Brolo, R. Nirwan, Donna Hohertz, Jamie K. Scott, Samantha M. Grist, Reuven Gordon, C. Hui, Naveed Gulzar |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | IEEE Sensors Journal. 11:2732-2739 |
| ISSN: | 1558-1748 1530-437X |
| Popis: | Isolation of monoclonal (M-) antibodies (Abs) from Ab-secreting cells (ASCs) is impeded by difficulties in identifying the minority of ASCs secreting Ab(s) of interest. We present a novel label-free system that traps ASCs into biocompatible microwells and detects the binding of secreted Ab(s), to a surface-immobilized antigen (Ag), as induced shifts in the extraordinary optical transmission (EOT) spectra through milled nanohole arrays. In this paper, a biotinylated target Ag (Bio-HA and Bio-2F5) is exposed to varying known MAb concentrations (17/9 and 2F5). The mean EOT shift in response to MAb concentration is similar in shape to specific MAb-Ag binding as seen in an ELISA. In another experiment, hybridoma cells secreting 17/9 MAb are trapped in wells inset into poly(dimethyl siloxane) (PDMS) (5-20 cells/trap), to which the nanohole arrays of Bio-HA “Sample” and Bio-2F5 “Ag Control” slides are aligned. Simultaneously, a Bio-HA “Media Control” slide is exposed to MAb-free media. The mean “Sample” shift (3.2 ±0.1 nm) is distinguished from the mean “Ag Control” shift (1.2 ±0.4 nm), but indistinguishable from the mean “Media Control” shift (3.6 ±0.3 nm). We have made significant progress in detecting Ab(s) secreted from trapped ASCs. With further development, our device could identify ASCs of interest rapidly, streamlining therapeutic MAb discovery. |
| Databáze: | OpenAIRE |
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