Plant Regeneration From Mature Avena Tissue Explants

Autor: Allan Zipf, G. Hassan, Darrell E. Wesenberg, Govind C. Sharma
Rok vydání: 1999
Předmět:
Zdroj: Cereal Research Communications. 27:25-32
ISSN: 1788-9170
0133-3720
DOI: 10.1007/bf03543915
Popis: Successful plant transformation is dependent upon a reliable plant regeneration system. In cereals, immature embryos are the explants of choice but are inconvenient due to their temporal nature and maintenance requirements. The effects of three different auxins, 2,4-D, dicamba, and picloram, on two mature tissue explant types, mature embryo and hypocotyl, of three Avena genotypes, GAF/Park, Park, and 88Ab3073, were evaluated for callus formation, somatic embryogenesis (SE), and plant regeneration capabilities. Both mature embryo and hypocotyl explants were proliferated on modified Murashige and Skoog (MS) basal medium containing 5, 10, and 20 μM of each auxin and were regenerated on 1/2-MS medium without hormone. Although plant number was significantly correlated with SE number, it was not absolutely linked to a priori somatic embryogenesis, especially for 88AB3073 explants. While stimulating callus growth, both dicamba and picloram decreased embryogenicity and regenerability over that obtained with 2,4-D. Picloram may be useful for the isolation of SE-related genes as somatic embryogenesis in oat callus was strongly inhibited in the presence of picloram.
Databáze: OpenAIRE
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