| Autor: |
Mario Mellado, Rubén Barroso, Laura Martínez-Muñoz, Anabel Guedán Paredes, José Miguel Rodríguez-Frade |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781493923359 |
| DOI: |
10.1007/978-1-4939-2336-6_12 |
| Popis: |
The G protein-coupled receptors (GPCRs) form one of the largest membrane receptor families. The nature of the ligands that interact with these receptors is highly diverse; they include light, peptides and hormones, neurotransmitters, and small molecular weight compounds. The GPCRs are involved in a wide variety of physiological processes and thus hold considerable therapeutic potential.GPCR function is usually determined in cell-based assays, whose complexity nonetheless limits their use. The use of alternative, cell-free assays is hampered by the difficulties in purifying these seven-transmembrane domain receptors without altering their functional properties. Several methods have been proposed to immobilize GPCR on biosensor surfaces which use antibodies or avidin-/biotin-based capture procedures, alone or with reconstitution of the GPCR physiological microenvironment. Here we propose a method for GPCR immobilization in their native membrane microenvironment that requires no manipulation of the target receptor and maintains the many conformations GPCR can adopt in the cell membrane. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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